Thermo Fisher Scientific HSP27 Recombinant Rabbit Monoclonal Antibody (155)
Human HSP27 단백질을 인식하는 recombinant rabbit monoclonal 항체. Western blot, ICC/IF, Flow cytometry에 적합. 보존제가 없는 고순도 단백질 A 정제 제품으로, 높은 특이성과 재현성을 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:20–1:100 |
| Flow Cytometry (Flow) | 1:25–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 155 |
| Immunogen | Recombinant Human HSP27 protein (Met1–Lys205) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785217 |
Additional Formats
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Human HSP27.
Target Information
HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein that functions as a molecular chaperone, maintaining denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates biological processes including phosphorylation and axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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