Thermo Fisher Scientific PHC1 Monoclonal Antibody (1F3F3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1F3F3

Immunogen

Purified recombinant fragment of human PHC1 (AA: 758-1004) expressed in E. coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PHC1,uniProtId:P78364-1,ncbiNodeId:9606,antigenRange:758-1004,antigenLength:1004,antigenImageFileName:MA5-31842_PHC1_P78364-1_House_mouse.svg,antigenImageFileNamePDP:MA5-31842_PHC1_P78364-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787465

Product Specific Information

MA5-31842 has been tested in indirect ELISA.

Target Information

Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development. PHC1 (polyhomeotic homolog 1), also known as EDR1, HPH1 or RAE28, is a 1,004 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex. Specifically, the PcG PRC1 complex modifies histones, remodels chromatin and mediates monoubiquination of Histone H2A. Other constituent proteins involved in the PcG PRC1 complex are Mel-18, Bmi-1, M33, MPc2, MPc3, RING1, Ring1b, as well as several others. Existing as a homodimer, PHC1 contains one FCS-type zinc finger and a SAM (sterile alpha motif) domain. PHC1 is encoded by a gene located on human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.