
Thermo Fisher Scientific PDPK1 Monoclonal Antibody (3H3D9)
PDPK1 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 3H3D9). Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능. 고순도 Protein G 정제, 1 mg/mL 농도, PBS buffer 및 sodium azide 함유. 연구용으로만 사용.
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Thermo Fisher Scientific PDPK1 Monoclonal Antibody (3H3D9)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3H3D9 |
| Immunogen | Purified recombinant fragment of human PDPK1 (AA: 457–556 expressed in E. coli) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787458 |
Product Specific Information
MA5-31835 has been tested in indirect ELISA.
Target Information
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases and activates conventional PKC and PKC zeta through phosphorylation of key threonine residues. It also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), leading to activation of glycogen synthase and stimulation of glycogen synthesis. Due to its role in insulin-induced glycogen synthesis and PKC activation, PDPK1 is an important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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