Thermo Fisher Scientific Raf -A Polyclonal Antibody
Raf-A 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB 및 IHC(P) 분석에 적합합니다. 인간, 마우스, 랫트에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 안정화 용액에 보관되며, 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide of internal segment of human Raf-A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human cervix carcinoma and kidney tissues
- Cellular location: Transduction of mitogenic signals from the cell membrane to the nucleus
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all cell lines tested. It is the cellular homolog of v-Raf, the transforming gene product of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of v-Raf is associated with transformation and mitogenesis, while Raf-1 activity is normally suppressed by a regulatory N-terminal domain.
A-Raf, another member of the Raf gene family, shows substantial homology to Raf-1 within the kinase domain but less elsewhere. A-Raf expression is highest in urogenital tissues and kidney, and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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