Thermo Fisher Scientific CD4 Monoclonal Antibody (OX-35)
CD4 단백질을 인식하는 OX-35 클론의 마우스 단클론 항체로, Rat 시료에 반응합니다. Flow Cytometry, IHC, ICC, IP 등 다양한 응용에 적합합니다. Protein A로 정제된 액상 항체이며, 1 mg/mL 농도로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 1:10–1:50 | 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OX-35 |
| Immunogen | MLR generated rat T helper lymphocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_928270 |
Product Specific Information
This clone recognizes a different epitope on the CD4 molecule than the W3/25 clone.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and acts as a co-receptor for HIV. CD4 is mainly expressed on T helper lymphocytes and can also be found on monocytes, macrophages, and dendritic cells.
At the tissue level, CD4 expression is observed in thymus, lymph nodes, tonsils, spleen, and in specific regions of the brain and gut.
CD4 functions to initiate or augment early T-cell activation via association with the T-cell receptor complex and protein tyrosine kinase Lck. It may also mediate neuronal damage in infectious and immune-mediated CNS diseases.
Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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