Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), APC, eBioscience
Mouse CD45.1 단백질을 인식하는 A20 클론 단일클론 항체로, APC 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 가능합니다. Leukocyte common antigen 분석 및 면역학 연구용으로 적합합니다.
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Thermo Fisher Scientific CD45.1 Monoclonal Antibody (A20), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | 108 publications |
| Functional Assay (Functional) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A20 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469397 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506
- FITC
- NovaFluor Blue 510, 610-30S, 660-40S
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 702
- Request custom conjugation available
Product Specific Information
The A20 monoclonal antibody reacts with the mouse CD45 molecule (leukocyte common antigen, LCA) in CD45.1-expressing mouse strains such as SJL/J, DA, STS/A, and RIII. CD45.1 is expressed by all leukocytes in these strains.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested by flow cytometric analysis of mouse splenocytes
- Recommended usage: ≤ 0.5 µg per test (100 µL final volume, 10^5–10^8 cells/test)
- Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of surface proteins in lymphocytes. It regulates Src-family kinases and plays a key role in lymphocyte development and antigen signaling.
CD45 exists in multiple isoforms due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic domains. Isoform expression varies by cell type and physiological state. CD45 also modulates integrin-mediated signaling and DNA fragmentation during apoptosis.
CD45RA is one isoform with restricted expression among lymphoid cell subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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