Thermo Fisher Scientific His Tag Monoclonal Antibody (6G2A9), HRP, THE

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

-

ELISA (ELISA)

0.05-0.5 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 8 publications 8 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

6G2A9

Immunogen

A synthetic peptide HHHHHH coupled to KLH

Conjugate

HRP HRP HRP

View additional formats

• Unconjugated
• Biotin
• FITC
• iFluor 488
• iFluor 555
• iFluor 647
• Request custom conjugation

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.01% thimerosal

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.

GenScript THE™ His Tag Antibody [HRP], mAb, Mouse is HRP conjugated THE™ Anti-His Monoclonal Antibody (Mouse) (A00186) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.

Target Information

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.