Thermo Fisher Scientific His Tag Monoclonal Antibody (6G2A9), HRP, THE

Applications and Tested Dilution

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Product Specifications

Additional Formats

• Unconjugated
• Biotin
• FITC
• iFluor 488
• iFluor 555
• iFluor 647
• Request custom conjugation

Product Specific Information

Reconstitute the lyophilized antibody with deionized water to a final concentration of 0.5 mg/mL.
GenScript THE™ His Tag Antibody [HRP], mAb, Mouse is HRP-conjugated THE™ Anti-His Monoclonal Antibody (Mouse) (A00186), purified from mouse ascites using a Protein A affinity column.
This antibody is suitable for detecting His fusion proteins or His tags in various immunoassays.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at either the N- or C-terminus of recombinant proteins to enable purification via immobilized metal affinity chromatography (IMAC).

Epitope tagging allows detection of recombinant proteins lacking specific antibodies by fusing a known epitope sequence.
His-tagged proteins are purified through metal chelate chromatography due to the histidine cluster’s affinity for metal ions such as Ni²⁺.
His tag-specific antibodies are widely used for detection and co-immunoprecipitation of tagged proteins.

⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.