Thermo Fisher Scientific His Tag Monoclonal Antibody (6G2A9), Biotin, THE
Biotin으로 결합된 Thermo Fisher Scientific His Tag Monoclonal Antibody (6G2A9)은 단백질의 His 태그 검출에 적합합니다. Western blot 및 ELISA에 최적화되어 있으며, 고순도 Protein A 정제 제품입니다. 재조합 단백질의 정량 및 검증 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1.0 µg/mL |
| ELISA | 0.02–0.2 µg/mL |
| Miscellaneous PubMed | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6G2A9 |
| Immunogen | A synthetic peptide (HHHHHH) coupled to KLH |
| Conjugate | Biotin |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
GenScript THE™ His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated form of THE™ His Tag Antibody (A00186), purified from mouse ascites using Protein A affinity chromatography.
This antibody is suitable for detecting the expression level of His in fusion proteins.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
His tags are commonly expressed at either the N- or C-terminal regions of recombinant proteins to facilitate purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging enables detection of recombinant proteins even when specific antibodies are unavailable.
His-tagged proteins can be purified using Ni²⁺-NTA adsorbents due to the strong and selective affinity between histidine clusters and nickel ions.
His tag-specific antibodies are widely used for detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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