Thermo Fisher Scientific His Tag Monoclonal Antibody (6G2A9), Biotin, THE

Applications and Tested Dilution

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Product Specifications

Additional Formats

• Unconjugated
• FITC
• HRP
• iFluor 488
• iFluor 555
• iFluor 647
• Request custom conjugation

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
GenScript THE™ His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated form of THE™ His Tag Antibody (A00186), purified from mouse ascites using Protein A affinity chromatography.
This antibody is suitable for detecting the expression level of His in fusion proteins.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
His tags are commonly expressed at either the N- or C-terminal regions of recombinant proteins to facilitate purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging enables detection of recombinant proteins even when specific antibodies are unavailable.
His-tagged proteins can be purified using Ni²⁺-NTA adsorbents due to the strong and selective affinity between histidine clusters and nickel ions.
His tag-specific antibodies are widely used for detection or co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.