Thermo Fisher Scientific CD25 Chimeric Recombinant Rabbit Monoclonal Antibody (2E4)
마우스 CD25를 인식하는 키메릭 재조합 래빗 모노클로날 항체로, WB, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능. IL-2 결합 부위를 방해하지 않으며, 고순도의 Protein A 정제 항체. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Functional Assay | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2E4 |
| Immunogen | The original version of this antibody was raised to recognize mouse CD25. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074794 |
Product Specific Information
Specificity: Clone 2E4 recognizes mouse CD25. This epitope is distinct from the IL-2 binding site and does not block binding of IL-2 to CD25.
Target Information
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine receptor involved in the proliferation of T and B lymphocytes. The IL2 receptor complex consists of IL2RA, IL2RB, and IL2RG subunits, forming a high-affinity receptor.
Homodimeric IL2RA chains result in low-affinity binding, while IL2RB homodimers yield medium affinity. IL2 expression in mature thymocytes is monoallelic, representing a unique regulatory mechanism.
IL2 is primarily produced by mature T cells and functions as a growth factor, differentiation factor, and apoptosis regulator. It promotes B cell proliferation, enhances NK cell activity, and inhibits granulocyte-macrophage colony formation.
Disruption of the corresponding gene in mice leads to colitis-like disease, suggesting a role in immune regulation. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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