Thermo Fisher Scientific ETS1 Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:3,000

Product Specifications

Product Specific Information

• Positive Control: Jurkat, Jurkat nuclear extract
• Predicted Reactivity: Rat (100%), Rabbit (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); ETS-1; EWSR2; p54) is a 441 amino acid protein belonging to the DNA-binding ETS family.
It is a multifunctional protein with:

• N-terminal pointed domain
• Central transactivation domain
• Two auto-inhibitory domains flanked by conserved C-terminal Ets domain

ETS1 interacts with PEBP2alphaA, p300/CBP, and mediates transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase.
Activated by Ras/MAPK signaling, its expression increases in resting T-cells.
Activity is modulated by AP-1, Sp-1, c-myb, and MafB.
Sp100 activates ETS1 in nuclear bodies, while EAP1/Daxx interaction represses MMP1 and BCL2 transactivation.
ETS1 also interacts with STAT6 to modulate Socs-1 expression induced by IL-4 and with GATA3 to positively regulate Tax-1 dependent IL-5 expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.