Thermo Fisher Scientific CD4 Monoclonal Antibody (W3/25), FITC
Rat CD4 단백질을 인식하는 W3/25 클론의 단클론 항체로, FITC 형광 표지되어 있습니다. Flow cytometry, ICC/IF, IHC(P) 등 다양한 응용에 적합하며, Protein G로 정제된 고순도 항체입니다. 4°C 보관, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 0.5 µg/1×10⁶ cells | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | W3/25 |
| Immunogen | Rat thymocyte membrane |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 4–5 mg/mL BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538780 |
Available Formats
- Unconjugated (Cat. MA5-17387)
- Biotin (Cat. MA5-17388)
- PE (Cat. MA5-17392)
- Custom conjugation available upon request
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and serves as a co-receptor for HIV. CD4 is primarily expressed in T helper cells but may also be found in monocytes, macrophages, and dendritic cells. It is detected in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. CD4 functions in T-cell activation via association with the T-cell receptor complex and Lck kinase. It may also mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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