Thermo Fisher Scientific ATG5 Monoclonal Antibody (ATG5, 2492)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Tested in direct ELISA (for coating use antibody at 1–5 µg/mL).
• For Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

ATG5 (Autophagy Related 5) is a key component of the autophagy pathway and may play an important role in apoptosis. It is involved in several cellular processes including:

• Mitochondrial quality control after oxidative damage
• Negative regulation of innate antiviral immune response
• Lymphocyte development and proliferation
• MHC II antigen presentation
• Adipocyte differentiation

Following conjugation to ATG12, the ATG5–ATG12 complex participates in autophagosome formation and contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5–ATG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and permitting viral replication.
Dysfunction of ATG5 is associated with diseases such as spinocerebellar ataxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.