Thermo Fisher Scientific MDM2 Monoclonal Antibody (OTI1B4), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating p53 stability. It is located on chromosome 12q.
Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 enhances its interaction with p300, promoting MDM2-mediated ubiquitination and degradation of p53.
Phosphorylation also inhibits binding to p19ARF, further increasing p53 degradation. MDM2 negatively regulates p53 function by inhibiting its transactivation and promoting degradation through its E3 ubiquitin ligase activity.
Overexpression of MDM2 can inactivate p53, reducing its tumor suppressor function. MDM2 is implicated in apoptosis and cell cycle regulation and is overexpressed in various human malignancies, including soft tissue carcinomas and breast cancer.
It also interacts with proteins such as retinoblastoma 1 and ribosomal protein L5. More than 40 alternatively spliced transcript variants of MDM2 have been identified in tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.