Thermo Fisher Scientific SOS1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human SOS1. The exact sequence is proprietary. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SOS1,uniProtId:Q07889-1,ncbiNodeId:9606,antigenRange:1333,antigenLength:1333,antigenImageFileName:PA5-78174_SOS1_Q07889-1_Rabbit.svg,antigenImageFileNamePDP:PA5-78174_SOS1_Q07889-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.58 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2736647

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2

Predicted Reactivity: Mouse (100%), Rat (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Son of sevenless (SOS) is a guanine nucleotide exchange factor that activates Ras in response to growth factor stimulation. Sos1, and its closely related Sos2 paralog, are comprised of an N-terminal histone-binding domain, a RacGEF catalytic (DH) domain juxtaposed with a PH domain, a Ras exchanger motif (REM) domain, a RasGEF catalytic domain, and a C-terminal proline-rich region. Sos1 catalyzes the GDP-GTP exchange within the membrane-bound GTPase Ras and thereby switches on a key signaling circuit that involves the activation of MAPK cascade central to cellular proliferation, survival and differentiation. Growth factor stimulation rapidly induces the phosphorylation of Sos on multiple serine and threonine sites. Phosphorylation of residues within the Sos C-terminus by the MAP/ERK kinase disrupts the Grb2-Sos complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.