Thermo Fisher Scientific Phospho-BAD (Ser91, Ser128) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of Serine 91 (E-P-S(p)-P-F) derived from Human BAD or Serine 128 (E-E-E-L-S(p)-P-F-R-G) derived from Mouse BAD. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BAD,uniProtId:Q92934-1,ncbiNodeId:9606,antigenRange:91,antigenLength:168,antigenImageFileName:PA5-37484_BAD_Q92934-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37484_BAD_Q92934-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:BAD,uniProtId:Q61337-1,ncbiNodeId:10090,antigenRange:128,antigenLength:204,antigenImageFileName:PA5-37484_BAD_Q61337-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37484_BAD_Q61337-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2554093

Product Specific Information

A suggested positive control for Western blot is COS7 cells; suggested positive control for IHC is human breast carcinoma.

Target Information

The protein encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.