
Thermo Fisher Scientific HLA-G Monoclonal Antibody (87G), Biotin
HLA-G 단백질의 막 결합형 및 용해형 형태를 모두 인식하는 mouse monoclonal antibody. Biotin 결합 형식으로 제공되며, IHC, ICC, Flow cytometry, ELISA 등에 사용 가능. Human 시료에 반응하며, 4°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1–3 µg/mL | - |
| ELISA | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 87G |
| Immunogen | HLA-B27 transgenic mice were immunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin. |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152093 |
Available Conjugate Formats
Product Specific Information
This antibody recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5).
HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and is expressed on the surface of trophoblast cells.
It will not cross-react with mouse or rat.
Target Information
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane and is approximately 45 kDa.
The HLA-G gene contains 8 exons:
- Exon 1 – leader peptide
- Exons 2 & 3 – alpha1 and alpha2 domains (peptide binding)
- Exon 4 – alpha3 domain
- Exon 5 – transmembrane region
- Exon 6 – cytoplasmic tail
HLA and MHC antibodies play a significant role in immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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