Thermo Fisher Scientific CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.5 µg/test
  View 14 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• FITC
• Functional Grade
• PE
• Request custom conjugation

Product Specific Information

The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein, a critical component of the T-cell receptor (TCR) complex and a marker of T-cell lineage. CD3 participates in signal transduction from the TCR to the nucleus, inducing IL-2 transcription and clonal expansion.

• In vitro: Immobilized G4.18 activates T cells; soluble form inhibits allogeneic responses and cytotoxicity.
• In vivo: Demonstrated to induce long-term specific tolerance to organ allografts.
• Applications Reported: Flow cytometric analysis.
• Applications Tested: Tested on rat splenocytes at ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorochrome Characteristics

• PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
• Excitation: 488 nm (Blue Laser)
• Emission: 710 nm
• Recommended filters: 685 LP dichroic mirror and 710/40 band pass (695/40 acceptable alternative).
• Stable with 2% formaldehyde fixation overnight at 4°C.
• 0.2 µm post-manufacturing filtered.

Target Information

CD3 is a subunit complex essential for transducing antigen-recognition signals in T cells and regulating TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits—structurally related immunoglobulin superfamily members encoded on human chromosome 11.

• Mediates signal transduction via double tyrosine-based motifs.
• Expressed on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells.
• Absent on B and NK cells.
• Decreased T lymphocyte percentages may occur in autoimmune diseases.
• CD3 gene defects are associated with CD3 immunodeficiency.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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