
Thermo Fisher Scientific Ephrin-A1 (extracellular) Polyclonal Antibody
Ephrin-A1 단백질의 세포외 도메인을 인식하는 Rabbit Polyclonal 항체. Western blot, ICC/IF, Flow cytometry에 사용 가능. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제됨. 동결건조 형태로 제공되며 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)KITHSPQAHDNPQEK, corresponding to amino acid residues 150–164 of human Ephrin-A1 (extracellular domain) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution:
Reconstitute with 25 µL, 50 µL, or 0.2 mL of double-distilled water (DDW), depending on sample size.
The antibody is shipped as a lyophilized powder at room temperature.
Upon arrival, store at -20°C.
Reconstituted solution can be stored at 4°C for up to 1 week.
For long-term storage, aliquot and keep at -20°C.
Avoid multiple freeze/thaw cycles.
Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
This gene encodes a member of the ephrin family. The ephrins and EPH-related receptors form the largest subfamily of receptor protein-tyrosine kinases and are involved in developmental processes, particularly in the nervous system and erythropoiesis.
Ephrins are divided into two classes:
- Ephrin-A class: anchored to the membrane by a glycosylphosphatidylinositol linkage
- Ephrin-B class: transmembrane proteins
The Ephrin-A1 (EFNA1) protein binds to EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants encoding different isoforms have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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