
Thermo Fisher Scientific EphA2 (extracellular) Polyclonal Antibody
EphA2 단백질의 세포외 영역을 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC(F), Flow cytometry 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 암 연구 및 세포 신호 전달 분석에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:300 |
| Flow Cytometry (Flow) | 2.5 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)HYLTAVGMGAKVELR, corresponding to amino acid residues 333–347 of human EphA2 (Extracellular, N-terminus) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution: Add 25 µL, 50 µL, or 0.2 mL of double-distilled water (DDW) depending on sample size.
The antibody ships lyophilized at room temperature and should be stored at -20°C upon arrival.
Reconstituted solution can be stored at 4°C, protected from light, for up to 1 week.
For longer storage, aliquot and keep at -20°C. Avoid repeated freeze-thaw cycles.
Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
EPHA2 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family.
These receptors are involved in developmental processes, particularly in the nervous system.
EPHA2 is overexpressed in many human cancers and plays a role in cell-cell interaction regulation, including differentiation, adhesion, motility, and apoptosis.
There are approximately 90 tyrosine kinase genes identified — 58 receptor-type (e.g., EGFR, EPH, FGFR, PDGFR, TRK, VEGFR families) and 32 cytosolic-type (e.g., ABL, FAK, JAK, SRC families).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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