
Thermo Fisher Scientific Ephrin-A2 (extracellular) Polyclonal Antibody
Ephrin-A2 단백질의 세포외 영역을 인식하는 토끼 폴리클로날 항체로, Western blot, IHC(F), Flow Cytometry에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200 |
| Flow Cytometry (Flow) | 5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)HYGAPLPPAERMER, corresponding to amino acid residues 71–84 of mouse Ephrin-A2 (extracellular region) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution:
Reconstitute with 25 µL, 50 µL, or 0.2 mL double distilled water (DDW), depending on sample size.
The antibody ships as a lyophilized powder at room temperature.
Upon arrival, store at -20°C.
The reconstituted solution can be stored at 4°C for up to 1 week.
For longer storage, aliquot and store at -20°C.
Avoid multiple freeze/thaw cycles.
Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date. Although their precise biological functions are not fully defined, they are implicated in central nervous system function and developmental processes.
Human and homologous receptors include EphA1–EphA8 and EphB1–EphB6.
Ligands for these receptors include ephrin-A and ephrin-B family members, such as ephrin-A2 (Elf-1), which acts as a ligand for EphA4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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