Thermo Fisher Scientific Phospho-Vimentin (Ser56) Recombinant Rabbit Monoclonal Antibody (7H18L9)
인간 Vimentin Ser56 인산화 부위를 인식하는 재조합 토끼 단클론 항체로, WB 및 ICC/IF에 적합합니다. 높은 특이성과 재현성을 제공하며, 로트 간 일관성이 우수합니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7H18L9 |
| Immunogen | Peptide corresponding to Human VIM (aa 53–61) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2633000 |
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Pig, Sheep, and Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned and expressed, and the best clones are selected for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular protein transport between the nucleus and plasma membrane. During early cellular development, vimentin is co-expressed with desmin and later replaced by tissue-specific IFPs. It also plays a role in steroid synthesis by mediating cholesterol transport within cells.
Phosphorylation of vimentin at Ser56 leads to filament disassembly and cytoskeletal rearrangements that facilitate lipid droplet–mitochondria interactions, enhancing steroidogenic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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