Thermo Fisher Scientific CD4 Recombinant Rabbit Monoclonal Antibody (ST0488)
Thermo Fisher Scientific의 CD4 Recombinant Rabbit Monoclonal Antibody (ST0488)은 인간 CD4 단백질을 인식하는 고특이성 항체로, WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. 일관된 로트 간 재현성과 높은 민감도를 제공하며, 연구용으로 최적화된 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | – |
| Immunohistochemistry (IHC) | – | 2 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400–1:800 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | – |
| Flow Cytometry (Flow) | – | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ST0488 |
| Immunogen | Recombinant protein within Human CD4 (aa 196–416) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809455 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and serves as a co-receptor for HIV. It is primarily expressed in T helper cells but also found in monocytes, macrophages, and dendritic cells.
At the tissue level, CD4 expression is detected in thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut.
CD4 functions to initiate or enhance early T-cell activation via association with the T-cell receptor complex and protein tyrosine kinase Lck. It may also mediate neuronal damage in infectious and immune-mediated CNS diseases.
Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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