Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), Brilliant Violet 711, eBioscience
마우스 NKp46(CD335)을 인식하는 29A1.4 클론 단일클론 항체로, Brilliant Violet 711 형광체 결합 제품입니다. Flow cytometry에 최적화되어 있으며, NK 세포 활성화 연구용으로 적합합니다. 포토산화에 민감하므로 암조건 보관이 필요합니다.
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Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), Brilliant Violet 711, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet™ 711, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 29A1.4 |
| Conjugate | Brilliant Violet™ 711 |
| Excitation / Emission Max | 406 / 711 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925581 |
Additional Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation available
Product Specific Information
The monoclonal antibody 29A1.4 recognizes mouse NKp46 (CD335), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a glycoprotein with two Ig-like domains and a short cytoplasmic tail, expressed uniquely on NK cells, including immature NK cells. It allows discrimination between NKT cells and NK cells (NKp46+, CD3-). Staining is not strain-specific and has been confirmed in C57Bl/6, SJL, CBA/CA, and BALB/C strains.
NKp46 plays a role in NK cell-mediated lysis of tumor and pathogen-infected cells. The 29A1.4 antibody activates NK cells in vitro but does not deplete them in vivo.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Cell count may range from 10^5–10^8 cells/test. Titrate for optimal performance.
Brilliant Violet™ 711 (BV711) emits at 713 nm and is suitable for cytometers with a 405 nm violet laser. Protect from light due to photo-induced oxidation sensitivity.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). BV711 is not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 713 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor contributing to NK cell efficiency in tumor cell lysis. It consists of two Ig-like domains assembled to leukocyte immunoglobulin-like and killer inhibitory receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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