Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation available

Product Specific Information

The monoclonal antibody 29A1.4 recognizes mouse NKp46 (CD335), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a glycoprotein with two Ig-like domains and a short cytoplasmic tail, expressed uniquely on NK cells, including immature NK cells. It allows discrimination between NKT cells and NK cells (NKp46+, CD3-). Staining is not strain-specific and has been confirmed in C57Bl/6, SJL, CBA/CA, and BALB/C strains.

NKp46 plays a role in NK cell-mediated lysis of tumor and pathogen-infected cells. The 29A1.4 antibody activates NK cells in vitro but does not deplete them in vivo.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Cell count may range from 10^5–10^8 cells/test. Titrate for optimal performance.

Brilliant Violet™ 711 (BV711) emits at 713 nm and is suitable for cytometers with a 405 nm violet laser. Protect from light due to photo-induced oxidation sensitivity.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). BV711 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 713 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor contributing to NK cell efficiency in tumor cell lysis. It consists of two Ig-like domains assembled to leukocyte immunoglobulin-like and killer inhibitory receptors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지