Thermo Fisher Scientific EpCAM/CD326 (Epithelial Marker) Polyclonal Antibody
EpCAM/CD326 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간 시료에 반응. Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용 가능. Recombinant human TACSTD1 항원을 이용해 제작되었으며, 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant human TACSTD1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Does not react with Mouse, Rat. Others not known.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in epithelial cells with a molecular weight of approximately 40 kDa. It functions as a calcium-independent homotypic adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cyclin A/E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for tumors of epithelial lineage.
Ep-CAM is found on the basolateral surface of most simple epithelia and many carcinoma types and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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