Thermo Fisher Scientific Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Violet 711, eBioscience
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Flow cytometry용 Rat IgG2b kappa 동종대조 항체로, Brilliant Violet 711 형광체 결합. 비특이적 결합 평가 및 실험 대조군 설정에 적합. Violet laser(405 nm) 기반 장비에 최적화. 4°C 암소 보관, 동결 금지.
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Applications
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eB149/10H5 |
| Conjugate | Brilliant Violet™ 711 |
| Excitation / Emission Max | 406 / 711 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925583 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV (395–805) series
- Brilliant Violet (421–786) series
- eFluor (450–660) series
- FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright (436–780) series
- Functional Grade
- Custom conjugation available
Product Specific Information
- Description: Rat IgG2b kappa monoclonal antibody useful as an isotype control immunoglobulin.
- Applications Reported: Flow cytometric analysis.
- Applications Tested: Tested on human peripheral blood and mouse bone marrow cells. Use at same concentration as experimental antibody.
- Fluorochrome: Brilliant Violet™ 711 (BV711) emits at 713 nm, optimized for cytometers with a 405 nm violet laser.
- Buffer Recommendation: When using multiple polymer dyes (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
- Light Sensitivity: Protect from light to avoid photo-induced oxidation.
- Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C. Not compatible with methanol-based fixation.
- Excitation: 407 nm
- Emission: 713 nm
- Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
Rat IgG2b는 쥐 항체의 주요 아형 중 하나로, 병원체 중화 및 옵소닌 작용을 통해 면역 반응에 중요한 역할을 합니다.
연구용으로 면역조직화학, 유세포분석(Flow Cytometry), Western blotting 등에 널리 사용됩니다.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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