Thermo Fisher Scientific SFTPD Monoclonal Antibody (12g5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

1:800

View 2 publications 2 publications

ELISA (ELISA)

1:250

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Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

12g5

Immunogen

Recombinant neck CRD SP-D.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.03 mg/mL

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_1087629

Product Specific Information

HYB 245-01-02 is specific for human SP-D.

HYB 245-01-02 has been successfully used in Immunohistochemistry, ELISA and Western blot procedures. In immunohistochemical staining on paraffin embedded tissues, HYB 245-01 reacts specifically with SP-D.

Immunoreactivity can be found in epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder. In Western blotting after SDS-PAGE HYB 245-01 reacts strongly with SP-D, both in reduced and non-reduced forms. HYB 245-01 can be used for ELISA, but generally gives a weak signal.

The HYB 245-01-02 immunogen is recombinant neck CRD SP-D.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular head regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.