Thermo Fisher Scientific SFTPD Monoclonal Antibody (10B2), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

ELISA (ELISA)

1:2,000

-

Product Specifications

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

10B2

Immunogen

Native human SP-D purified from late pregnancy amniotic fluid.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_1087627

Product Specific Information

HYB 246-04B-01 is specific for SP-D from amniotic fluid, serum and bronchioalveolar lavage. HYB 246-04B-02 does not influence SP-D`s binding to carbohydrates.

HYB 246-04B-01 has been successfully used in ELISA and Western blot procedures. HYB 246-04B-01 is suitable as detection antibody in sandwich ELISA in combination with a polyclonal antibody against SP-D. Binding of HYB 246-04B-01 is Ca++ dependent. In Western blotting after SDS-PAGE HYB 246-04B-01 reacts with non-reduced SP-D only.

The HYB 246-04B-01 immunogen is native human SP-D purified from late pregnancy amniotic fluid.

Epitope of HYB 246-04B-01 is localised within the C-terminal neck CRD (carbohydrate recognition domain) of SP-D.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular head regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.