Thermo Fisher Scientific Apolipoprotein B Monoclonal Antibody (F2C3)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• MA5-14724 targets Apolipoprotein B in ELISA, IP, and RIA applications and shows reactivity with human samples.
• Immunogen: Purified human serum LDL.
• Detects Apolipoprotein B with a predicted molecular weight of approximately 513 kDa.
• Product MA514724 is a smaller package size of MIA1605 (formerly sold as a Seradyn product).

Target Information

Apolipoprotein B (apo B) in human plasma is a major protein of low-density lipoproteins (LDL) with a molecular mass of approximately 260–500 kDa.
Apolipoprotein B binds to specific receptors on cell membranes and is involved in removal of LDL and very low-density lipoprotein (VLDL) cholesterol from circulation.
LDL is the carrier protein for cholesterol in the blood and mediates uptake and clearance of cholesterol via receptor binding.

In atherosclerotic lesions, oxidatively modified LDL is found and is recognized and ingested by macrophages via scavenger receptor A and CD36.
Apolipoprotein B is synthesized mainly in the liver and is a major apolipoprotein of VLDL, IDL, and LDL, as well as a component of lipoprotein(a).
It is a ligand for the LDL receptor, and elevated levels are associated with premature atherosclerosis.
Normal plasma apolipoprotein B levels are around 800 mg/L.

Apolipoprotein B occurs as two main isoforms, apoB-48 and apoB-100, encoded by a single gene. The shorter apoB-48 is produced by RNA editing of apoB-100 transcript, introducing a stop codon at residue 2180.
Mutations in the gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective Apolipoprotein B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.