Thermo Fisher Scientific TLR7 Polyclonal Antibody
TLR7 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능하며, Protein G로 정제된 액상 형태입니다. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | 1 publication |
| Immunohistochemistry (Frozen) (IHC-F) | 1:5–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:5–1:1,000 | - |
| Flow Cytometry (Flow) | 2–5 µg / 10⁶ cells | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 706–749 of human TLR7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1961065 |
Product Specific Information
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
- This antibody will cross-react with mouse TLR7.
- In human Ramos cells, additional bands are seen.
Target Information
TLR7 is a highly conserved membrane protein belonging to the Toll-like receptor (TLR) family, containing 27 leucine-rich repeats (LRR) and a TIR domain. It plays a key role in pathogen recognition and activation of innate immunity. TLR7 is activated by single-stranded RNA viruses such as influenza and vesicular stomatitis virus (VSV). It is predominantly expressed in lung, placenta, and spleen, and is located near TLR8. TLR7 signals through MyD88 and TRAF6, leading to NF-kappaB activation, cytokine secretion, and inflammatory responses. It recognizes pathogen-associated molecular patterns (PAMPs) to mediate immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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