Thermo Fisher Scientific GSDMD Monoclonal Antibody (A8D7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

A8D7

Immunogen

Recombinant protein within human GSDMD aa 101-300/484. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GSDMD,uniProtId:P57764-1,ncbiNodeId:9606,antigenRange:101-300,antigenLength:484,antigenImageFileName:MA5-50595_GSDMD_P57764-1_House_mouse.svg,antigenImageFileNamePDP:MA5-50595_GSDMD_P57764-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3093477

Product Specific Information

Positive control: PC-3 cell lysate, NIH/3T3 cell lysate, THP-1 cell lysate.

Predicted band size: 53 kDa

Subcellular Location: Cytosol, Inflammasome; Secreted, Cell membrane.

Target Information

GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.