Thermo Fisher Scientific CD80 Monoclonal Antibody (16-10A1)
CD80 단백질을 인식하는 아르메니아 햄스터 유래 단클론 항체로, Flow Cytometry, IHC, IP 등 다양한 면역실험에 활용 가능. 비결합형 액상 형태이며, Protein A로 정제되어 높은 순도 제공. 항원제시세포의 CD80 발현 분석에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 1–4 µg/mL | 8 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Blocking Assay (BLOCK) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG2 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16-10A1 |
| Immunogen | CD80-transfected CHO cell line |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153728 |
Product Specific Information
This antibody reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of the immunoglobulin supergene family. CD80 is expressed on professional antigen-presenting cells such as dendritic cells, macrophages, and activated B lymphocytes.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands of the T-cell costimulatory molecule CD28 and the inhibitory receptor CTLA-4 (CD152). Both molecules are expressed on professional antigen-presenting cells and are essential for T-cell activation. CD80 is rapidly induced on the surface of activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes through binding to CD28 and CTLA-4. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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