Thermo Fisher Scientific Bim Polyclonal Antibody
Rabbit polyclonal antibody targeting human BIM protein. Validated for WB and IHC applications. Supplied as liquid, unconjugated form at 0.2 mg/mL concentration. Suitable for apoptosis-related research, stored at 4°C short term or -20°C long term.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from N-terminus of human BIM |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2549749 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is placenta tissue.
Target Information
BIM belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators involved in various cellular activities.
The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It interacts with other BCL-2 family members, including BCL2, BCL2L1/BCL-X(L), and MCL1, acting as an apoptotic activator.
Expression of this gene can be induced by nerve growth factor (NGF) and the forkhead transcription factor FKHR-L1, suggesting a role in neuronal and lymphocyte apoptosis.
Transgenic mouse studies indicate that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection.
Several alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
- PA5-32276_Bim_O43521-1_Rabbit.svg
- PA5-32276_Bim_O43521-1_Rabbit_PDP.jpeg
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