Thermo Fisher Scientific CX3CR1 Monoclonal Antibody (2A9-1), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CX3CR1 Monoclonal Antibody (2A9-1), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 5 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• Biotin
• PE
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 702
• Request custom conjugation available

Product Specific Information

This 2A9-1 monoclonal antibody reacts with human CX3CR1, the receptor for fractalkine, a transmembrane chemokine of the CX3C family. CX3CR1 is a seven transmembrane G protein-coupled receptor expressed on CD16+ NK cells, T cells (CD8+, CD4+, γ/δ), and monocytes. It is also found on osteoclast precursors and microglia, but shows little to no expression on B cells and granulocytes.
Fractalkine and CX3CR1 mediate cell-cell adhesion and chemotaxis of NK cells, T cells, and monocytes. Expression correlates with high levels of intracellular perforin and granzyme B.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometry of normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg)/test (in 100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.

PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5. It emits at 710 nm and is excited at 488 nm (blue laser). Recommended filters: 685 LP dichroic mirror with 710/40 band pass (695/40 acceptable alternative).

PerCP-eFluor® 710 conjugated antibodies are stable when samples are fixed with freshly prepared 2% formaldehyde overnight at 4°C.
Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CX3CR1 is a chemokine receptor acting as a coreceptor for HIV infection. Human, mouse, and rat CX3CR1 genes (V28, CMKBRL1, RBS11) encode a seven transmembrane protein identified as the receptor for fractalkine. CX3CR1 mediates leukocyte migration and adhesion, and is expressed in various human tissues and cell lines.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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