Thermo Fisher Scientific Tmem119 Monoclonal Antibody (V3RT1GOsz), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific Tmem119 Monoclonal Antibody (V3RT1GOsz), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 μg/test
• View 4 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Brilliant UV 805
• Brilliant Violet 421
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

This V3RT1GOsz monoclonal antibody recognizes mouse Tmem119 and is validated for flow cytometry.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse brain cells post-myelin removal; ≤ 0.25 μg/test recommended.
  A test = antibody amount staining cells in 100 µL volume.
  Cell number range: 10⁵–10⁸ cells/test.
  Optimize titration per assay.

Fluorochrome Information:
PerCP-eFluor 710 emits at 710 nm and is excited with a 488 nm blue laser.
Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
Check instrument compatibility.

Light Sensitivity:
Protect vial and stained samples from light; dye is photo-sensitive.

Fixation Guidance:
Samples may be stored in:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-57)
  for up to 3 days at 4°C in dark with minimal brightness loss.

Excitation / Emission / Laser:
488 nm / 710 nm / Blue Laser

Target Information

Tmem119 encodes a single-pass type I transmembrane protein interacting with microRNAs and transcription factors (SMAD1, SMAD5, RUNX2).
It serves as a microglial surface marker, distinguishing microglia from infiltrating macrophages.
Microglia are brain-resident phagocytes involved in debris clearance and neuronal pruning, exhibiting neuroprotective or neurotoxic roles depending on context.
Reactive microglia co-express Iba1 and contribute to neurodegenerative disease pathology (Alzheimer’s, Parkinson’s, Huntington’s, Multiple Sclerosis).
Outside the CNS, Tmem119 may play a role in osteoblast differentiation and ossification.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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