Thermo Fisher Scientific DARC Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

-

Flow Cytometry (Flow)

0.25 µg per million cells

-

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant mouse DARC Met1-Pro61, Ala115-Cys127, Ser186-Lys205, Tyr264-Asn285 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DARC,uniProtId:Q9QUI6-1,ncbiNodeId:10090,antigenRange:1-61|115-127|186-205|264-285,antigenLength:334,antigenImageFileName:PA5-47861_DARC_Q9QUI6-1_Sheep.svg,antigenImageFileNamePDP:PA5-47861_DARC_Q9QUI6-1_Sheep_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576815

Product Specific Information

Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.

Target Information

ACKR1 or DARC is an atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades resulting instead in chemokine sequestration, degradation, or transcytosis. ACKR1 is also known as an interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. It has a promiscuous chemokine-binding profile by interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. ACKR1 also acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1, TARC and for the malaria parasites P.vivax and P.knowlesi.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.