Thermo Fisher Scientific B3GNT1 Polyclonal Antibody
B3GNT1 단백질을 인식하는 Thermo Fisher Scientific의 sheep polyclonal 항체로, 인간 및 마우스 시료에 반응합니다. Western blot에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며, -20°C에서 보관합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHO-derived recombinant human beta-1,3-N-acetylglucosaminyltransferase 1 (B3GNT1) Asp43–Cys415 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610270 |
Product Specific Information
- In direct ELISAs, less than 1% cross-reactivity with recombinant human B4GalT1 and B3GNT2 is observed.
- Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
Beta-1,4-glucuronyltransferase involved in O-mannosylation of alpha-dystroglycan (DAG1).
Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE during synthesis of phosphorylated O-mannosyl glycan (PubMed:25279699, PubMed:25279697).
Phosphorylated O-mannosyl glycan is a carbohydrate structure present in alpha-dystroglycan (DAG1), required for binding laminin G-like domain-containing extracellular proteins with high affinity.
Required for axon guidance via its function in O-mannosylation of alpha-dystroglycan (DAG1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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