Thermo Fisher Scientific NGF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Neutralization (Neu)

Assay-dependent

-

Dot blot (DB)

1:2,000

-

Inhibition Assays (IA)

1:10,000

-

Immunodiffusion (IDF)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NGF 2.5S from male mouse submaxillary glands. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NGF,uniProtId:P01139-1,ncbiNodeId:10090,antigenRange:1-241,antigenLength:241,antigenImageFileName:PA1-24732_NGF_P01139-1_Rabbit.svg,antigenImageFileNamePDP:PA1-24732_NGF_P01139-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

6 mg/mL

Purification

IgG fraction

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_795041

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Specificity: May be used in monitoring for NGF (2.5S, ß and 7S).

Target Information

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.