Thermo Fisher Scientific beta Actin Recombinant Rabbit Monoclonal Antibody (0N2H8), HRP
Western blot 및 ELISA에 적합한 HRP 결합 베타 액틴 항체. 다양한 종(사람, 마우스, 랫, 피그 등)에 반응. 세포골격 및 단백질 발현 연구용 로딩 컨트롤로 활용. 고순도 정제 및 안정한 액상 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:50,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Pig, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 0N2H8 |
| Immunogen | Recombinant protein of human HRP-conjugated beta-Actin |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 0.76 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2912086 |
Product Specific Information
Positive test controls include: HeLa, HepG2, MCF7, Mouse liver, Mouse kidney, Mouse lung, Rat liver, Rat kidney, Rat lung.
The target is usually found in the following locations: Cytoplasm, cytoskeleton.
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein present in all cell types and plays a key role in cell motility, structure, and integrity.
The beta and gamma isoforms coexist in most cells as components of the cytoskeleton. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin) with a two-stranded helical structure.
Due to its ubiquitous expression, beta actin is widely used as a loading control in protein detection assays such as Western blotting. Antibodies to beta-actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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