Thermo Fisher Scientific beta Actin Recombinant Rabbit Monoclonal Antibody (4C4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:100,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:5,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Pig, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4C4

Immunogen

Recombinant protein of human beta-Actin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2912087

Product Specific Information

Positive test controls include: 293T, HeLa, Mouse brain, Mouse testis, Mouse lung, Rat brain, Rat testis, Rat lung. The target is usually found in the following locations: Cytoplasm, cytoskeleton.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.