Thermo Fisher Scientific Tubulin alpha-1B Recombinant Rabbit Monoclonal Antibody (8E8W9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500 - 1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50 - 1:200

-

Product Specifications

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2L5F2

Immunogen

A recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human Tubulin alpha-1B. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Tubulin alpha-1B,uniProtId:P68363-1,ncbiNodeId:9606,antigenRange:1-80,antigenLength:451,antigenImageFileName:MA5-42947_Tubulin_alpha-1B_P68363-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42947_Tubulin_alpha-1B_P68363-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2912088

Product Specific Information

Positive test controls include: HeLa, PC-12, A-549, NIH/3T3, U-251MG, Mouse brain, Rat brain.

Target Information

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.