Thermo Fisher Scientific Tubulin alpha-1B Recombinant Rabbit Monoclonal Antibody (8E8W9)
Recombinant Rabbit Monoclonal Antibody targeting Tubulin alpha-1B. Validated for WB, IHC, and ICC/IF applications. Expressed in HEK293 cells with high specificity and reproducibility. Supplied as unconjugated liquid form for research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 – 1:200 |
| Immunocytochemistry (ICC/IF) | 1:50 – 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2L5F2 |
| Immunogen | Recombinant fusion protein containing amino acids 1–80 of human Tubulin alpha-1B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.33 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2912088 |
Product Specific Information
Positive test controls include: HeLa, PC-12, A-549, NIH/3T3, U-251MG, Mouse brain, Rat brain.
Target Information
Microtubules are intracellular dynamic polymers composed of conserved alpha/beta-tubulin heterodimers and microtubule-associated proteins (MAPs). They exhibit intrinsic polarity with dynamic plus ends and less dynamic minus ends, essential for processes such as mitosis, meiosis, cell shape maintenance, and intracellular transport. Microtubules also enable cell movement via flagella and cilia. In mammalian cells, minus ends are anchored in microtubule organizing centers (MTOCs). GTP is crucial for tubulin dimer association and microtubule formation. Microtubule polymerization is reversible, and dynamic instability describes alternating growth and shortening at minus ends. Alpha-tubulin (~50 kDa) exists as soluble dimers or polymerized microtubules, encoded by multiple tubulin genes forming distinct classes (six in humans).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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