Thermo Fisher Scientific RFWD3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 724 and774 of Human ring finger and WD repeat domain 3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RFWD3,uniProtId:Q6PCD5-1,ncbiNodeId:9606,antigenRange:724-774,antigenLength:774,antigenImageFileName:A301-398A-M_RFWD3_Q6PCD5-1_Rabbit.svg,antigenImageFileNamePDP:A301-398A-M_RFWD3_Q6PCD5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Pig, Orangutan, Monkey, Gorilla and Chimpanzee

Target Information

E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.