Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Ultra Violet 661, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 480
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG with an apparent molecular weight of 50–80 kDa.
CD16 includes two genes:

• CD16A (FcγRIIIA): expressed in macrophages and NK cells
• CD16B (FcγRIIIB): expressed in neutrophils

Two polymorphisms, NA-1 and NA-2, affect phagocytic capacity. CD16 binds IgG immune complexes, showing preference for IgG1 and IgG3. Upon binding, CD16 triggers signaling cascades leading to ADCC, phagocytosis, degranulation, and proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted; tested on human peripheral blood cells at 5 µL (0.25 µg) per test.
A test is defined as the amount of antibody to stain a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for cytometers with a 355 nm ultraviolet laser. Confirm instrument compatibility before use.

Buffer Recommendations:
When using multiple polymer dyes (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)
  Store up to 3 days at 4°C in the dark with minimal impact on brightness.

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

CD16 (FcγRIII) encodes a receptor recognizing the Fc portion of IgG, mediating immune complex clearance and cellular cytotoxicity.

• Gene: FCGR3A (chromosome 1)
• Expression: NK cells (membrane-anchored), neutrophils (PI-anchored via FCGR3B)
• Clinical relevance: Mutations linked to immunodeficiency 20, herpes zoster susceptibility, systemic lupus erythematosus, and alloimmune neonatal neutropenia.
• Transcript variants: Multiple isoforms identified.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

제품 이미지