Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

Description:
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on leukocytes and platelets. CD31 mediates adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤1.0 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titer for best performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and requires an ultraviolet (355 nm) laser. Confirm compatibility with your cytometer.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (#SB-4401-42) or
• Brilliant Stain Buffer™ (#00-4409-75)
  to minimize non-specific polymer interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (#00-8222-49) or
• 1-step Fix/Lyse Solution (#00-5333-54).

Fluorescence Characteristics:

• Excitation: 350 nm
• Emission: 660 nm
• Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates. Powered by Sirigen™.

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs. It is expressed throughout the vascular system, mainly at intercellular junctions. CD31 mediates homophilic binding and plays roles in:

• Integrin-mediated adhesion
• Transendothelial migration
• Angiogenesis and apoptosis
• Regulation of immune signaling and autoimmunity
• Macrophage phagocytosis and thrombosis

For Research Use Only. Not for use in diagnostic procedures.

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