Thermo Fisher Scientific Cyclin B1 Polyclonal Antibody
Cyclin B1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, ICC/IF, Flow cytometry에 사용 가능. 세포주기 G2/M 전환 조절 연구에 적합하며, 항원 친화 크로마토그래피로 정제된 고순도 시약.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived human Cyclin B1 recombinant protein (Position: M1–V433) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746057 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein essential for control of the cell cycle at the G2/M transition. It forms a complex with p34 (cdc2) to create the maturation-promoting factor (MPF).
Two alternative transcripts exist — one constitutively expressed and one cell cycle-regulated, predominantly during G2/M phase. Cyclin B1 accumulates steadily during G2 phase and is abruptly destroyed at the end of mitosis via APC-dependent ubiquitination.
Cyclin B1 destruction is required for cell cycle progression and is overexpressed in various cancers including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: PA5-78941_Cyclin_B1_P14635-1_Rabbit.svg, PA5-78941_Cyclin_B1_P14635-1_Rabbit_PDP.jpeg)
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