Thermo Fisher Scientific CD1a Monoclonal Antibody (NA1/34-HLK)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Flow Cytometry (Flow)

1:50-1:100 (10 µL for 10^6 cells in 100 µL)

-

Product Specifications

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

NA1/34-HLK

Immunogen

Human thymocytes

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2073518

Product Specific Information

MA1-33801 detects CD1a from Cynomolgus monkey samples.

MA1-33801 has been successfully used in Flow cytometry and immunohistochemistry (frozen tissue) applications. Use 10 µL of the suggested working dilution to label 10^6 cells in 100 µL.

The MA1-33801 immunogen is human thymocytes.

Target Information

CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta 2 microglobulin. CD1a is expressed by cortical thymocytes, Langerhan`s cells and by interdigitating cells. CD1a is also expressed by some malignancies of T cell lineage and in histiocytosis X. CD1a is expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. CD1a is structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. CD1a localizes to the plasma membrane and recycles vesicles of the early endocytic system. At least five CD1 genes (CD1a, b, c, d, and e) are identified. Alternatively spliced transcript variants of CD1a have been observed, but their biological validity have not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.