Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), Biotin, eBioscience
HLA-A, B, C 복합체를 인식하는 W6/32 클론의 Biotin 결합 단일클론 항체로, 인간 세포의 MHC class I 항원을 검출에 적합. Flow cytometry, IHC, ICC 등 다양한 응용 가능. 고순도 Affinity chromatography 정제 및 안정적 PBS buffer 포맷.
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Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | View 10 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | W6/32 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467021 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Brilliant UV 395
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 650
- Brilliant Violet 786
- FITC
- Functional Grade
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 590
- NovaFluor Yellow 660
- NovaFluor Yellow 755
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
Description:
The W6/32 monoclonal antibody reacts with human MHC class I (HLA-A, B, C). These antigens, associated with beta 2-microglobulin, are expressed by all human nucleated cells and play a central role in cell-mediated immune response and tumor surveillance.
W6/32 mAb recognizes a non-polymorphic epitope shared among HLA-A, B, and C loci, immunoprecipitating both 43 kDa and 11–12 kDa chains. Cross-reactivity is observed in baboon, rhesus, and cynomolgus monkey.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended use ≤0.125 µg/test (in 100 µL volume). Cell number should be optimized (10⁵–10⁸ cells/test). Antibody titration recommended for best assay performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
HLA-class I MHC antigens are intrinsic membrane glycoproteins expressed on nucleated cells, noncovalently associated with beta2 microglobulin. They carry foreign determinants recognized by cytotoxic T cells, crucial for antiviral and antitumor defense.
Human HLA-class I antigens include HLA-A, HLA-B, and HLA-C molecules.
HLA and MHC antibodies are vital in Immunopeptidomics, enabling identification and characterization of neoantigens via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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