Thermo Fisher Scientific CD325 (N-Cadherin) Monoclonal Antibody (8C11), eBioscience
CD325(N-Cadherin)에 특이적인 8C11 클론 단일클론 항체로, 인간 시료에 반응합니다. WB, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능하며, 고순도(>90%)로 정제된 액상 형태입니다. 세포 접착 및 심장 발달 연구에 적합합니다.
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Thermo Fisher Scientific CD325 (N-Cadherin) Monoclonal Antibody (8C11), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-Dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 0.5 µg/test |
| Immunoprecipitation (IP) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8C11 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865059 |
| Purity | >90%, determined by SDS-PAGE |
| Aggregation | <10%, determined by HPLC |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugated Formats
- APC
- Biotin
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The 8C11 monoclonal antibody reacts with human CD325 (N-Cadherin), a 130 kDa member of the Cadherin superfamily composed of five extracellular repeats, a transmembrane domain, and a cytoplasmic domain. CD325-deficient mice exhibit embryonic lethality at day 10 of gestation with major cardiac and neural defects. CD325 is implicated in cardiac development, mesoderm formation, left-right symmetry establishment, and cardiac looping morphogenesis. Its expression is often dysregulated in cancer cells and is associated with cell cycle arrest.
Applications Reported:
Flow cytometric analysis, immunoprecipitation, Western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.
Applications Tested:
- Flow cytometry: ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
- Immunohistochemistry (FFPE): ≤10 µg/mL with low pH antigen retrieval
Titrate antibody for optimal performance.
Target Information
N-cadherin is a 140 kDa calcium-dependent cell adhesion glycoprotein of the cadherin family. It mediates homotypic cell-cell adhesion and plays a critical role in morphogenetic movements during development. Its function depends on interactions with the actin cytoskeleton via catenin proteins, regulated by β-catenin phosphorylation. N-cadherin consists of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail, essential for gastrulation and left-right asymmetry establishment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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