Thermo Fisher Scientific CD321 (F11R) Monoclonal Antibody (CSTEM27), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• PE
• Request custom conjugation

Product Specific Information

This monoclonal antibody CSTEM27 reacts with human CD321 (F11R, JAM-1, JAM-A).

Applications Reported:
This CSTEM27 antibody has been reported for use in immunocytochemical staining, flow cytometric analysis, and western blot. (Fluorochrome-conjugated CSTEM27 is recommended for use in flow cytometry.)

Applications Tested:

• Immunocytochemistry of adherent cells: ≤ 5 µg/mL
• Flow cytometric analysis of human iPSCs: 0.25 µg/test
  A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE
Aggregation: Less than 10%, as determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. It is a 32–41 kD glycoprotein with two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles as a homodimer and can associate with other membrane proteins, including integrins.

CD321 is expressed on leukocytes, platelets, erythrocytes, stem cells, epithelial and endothelial cells, and mediates cell-cell contact. It is located near epithelial tight junction components such as ZO-1 and occludin, and plays roles in maintaining epithelial and endothelial barriers, hematopoiesis, angiogenesis, and tumor proliferation, invasion, and metastasis.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.