Thermo Fisher Scientific Phospho-PKC alpha (Thr638) Recombinant Rabbit Monoclonal Antibody (ARC1591)
Phospho-PKC alpha (Thr638) 인산화 단백질을 특이적으로 인식하는 재조합 토끼 단클론 항체. Western blot 및 ELISA에 적합하며, 마우스와 랫트 시료에 반응. 고순도 친화 크로마토그래피 정제 및 안정한 액상 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1591 |
| Immunogen | A synthetic phosphorylated peptide around T638 of human PKC alpha (P17252) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.29 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849777 |
Product Specific Information
Immunogen sequence: VLTPP
Target Information
The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity.
PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca²⁺- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are involved in diverse cellular signaling pathways.
They also serve as major receptors for phorbol esters, a class of tumor promoters. Each member has a specific expression profile and distinct cellular role. PKC alpha is implicated in cell adhesion, transformation, cell cycle regulation, and cardiac contractility control based on knockout studies in mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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