Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Violet 786, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Violet 786, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–711)
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PerCP-Cyanine5.5
• Super Bright series (436–780)
• Custom conjugation available

Product Specific Information

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed on all hematopoietic cells except mature erythrocytes and platelets. Its cytoplasmic domain exhibits tyrosine phosphatase activity critical for lymphocyte activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤1.0 µg/test; 100 µL final volume)

Brilliant Violet™ 786 (BV786) is a tandem dye emitting at 786 nm, optimized for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution. BV786 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, constituting ~10% of lymphocyte surface proteins. It regulates Src-family kinases, T/B-cell receptor signaling, cytokine receptor pathways, and apoptosis. CD45 isoforms arise from alternative splicing and vary by cell type and physiological state.

For Research Use Only. Not for use in diagnostic procedures.

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