Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Violet 786, eBioscience
마우스 CD45 모든 아이소폼을 인식하는 30-F11 단클론 항체로, Brilliant Violet 786 형광체 결합. 유세포분석용으로 검증됨. 높은 특이성과 안정성, 405 nm 레이저 시스템에 최적화. 4°C 보관, 빛 차단 필요.
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Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Violet 786, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Violet™ 786 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 30-F11 |
| Conjugate | Brilliant Violet™ 786 |
| Excitation / Emission Max | 406 / 788 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925725 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780, Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–711)
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue, Red, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PerCP-Cyanine5.5
- Super Bright series (436–780)
- Custom conjugation available
Product Specific Information
The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed on all hematopoietic cells except mature erythrocytes and platelets. Its cytoplasmic domain exhibits tyrosine phosphatase activity critical for lymphocyte activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤1.0 µg/test; 100 µL final volume)
Brilliant Violet™ 786 (BV786) is a tandem dye emitting at 786 nm, optimized for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility before use.
When using multiple polymer dye-conjugated antibodies, use:
- Super Bright Complete Staining Buffer (Product # SB-4401-42)
- Brilliant Stain Buffer™ (Product # 00-4409-75)
Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution. BV786 is not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 786 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, constituting ~10% of lymphocyte surface proteins. It regulates Src-family kinases, T/B-cell receptor signaling, cytokine receptor pathways, and apoptosis. CD45 isoforms arise from alternative splicing and vary by cell type and physiological state.
For Research Use Only. Not for use in diagnostic procedures.
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